Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human GATA4 around the phosphorylation site of serine 105 (P-V-SP-P-R).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse
SwissProtNo:
P43694
Applications:
IF: 1:100~1:500 ELISA: 1:20000
Other Names:
DNA binding protein GATA-GT2, GAT4, GATA binding factor-4, GATA-4, Transcription factor GATA-4
Specificity Sensitivity:
GATA4 (Phospho-Ser105) antibody detects endogenous levels of GATA4 only when phosphorylated at serine 105.
References:
Huang W.Y., Gene 155:219-223(1995).
Garg V., e 424:443-447(2003).
All Sites:
Human: Ser105; Mouse: Ser105
P-peptide - +
Immunofluorescence analysis of HepG2 cells, using GATA4 (Phospho-Ser105) antibody.