• Catalog Number:

  E1A0934A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human GATA4 around the phosphorylation site of serine 262 (R-L-SP-A-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P43694

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000

• Other Names:

  DNA binding protein GATA-GT2, GAT4, GATA binding factor-4, GATA-4, Transcription factor GATA-4

• Specificity Sensitivity:

  GATA4 (Phospho-Ser262) antibody detects endogenous levels of GATA4 only when phosphorylated at serine 262.

• References:

  Audrey Holtzinger. Development, Sep 2005; 132: 4005 - 4014. José Rivera-Feliciano. Development, Sep 2006; 133: 3607 - 3618. Elisabeth M. Zeisberg. J. Clin. Invest., Jun 2005; 115: 1522 - 1531. Jakub Kwintkiewicz. Mol. Endocrinol., Apr 2007; 21: 933 - 947.

• All Sites:

  Human: Ser262; Mouse: Ser261; Rat: Ser261

Western blot analysis of extracts from 293 cells, using GATA4 (Phospho-Ser262) antibody.