Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human GATA3 around the phosphorylation site of serine 308 (R-L-SP-A-A).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse
SwissProtNo:
P23771
Applications:
IF: 1:100~1:500 ELISA: 1:10000
Other Names:
GATA-binding factor 3, Trans-acting T-cell specific transcription factor GATA-3
Specificity Sensitivity:
GATA3 (Phospho-Ser308) antibody detects endogenous levels of GATA3 only when phosphorylated at serine 308.
References:
Dorota Kurek, Development, Jan 2007; 134: 261 - 272.
M. Andrew Nesbit, J. Biol. Chem., May 2004; 279: 22624 - 22634.
Wei Xu, Blood, May 2007; 109: 4406 - 4414.
David Grote, Development, Jan 2006; 133: 53 - 61.
All Sites:
Human: Ser308; Mouse: Ser308
P-peptide - +
Immunofluorescence analysis of HuvEc cells, using GATA3 (Phospho-Ser308) antibody.