Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human FosB around the phosphorylation site of serine 27 (Y-L-SP-S-V).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse
SwissProtNo:
P53539
Applications:
IF: 1:100~1:500 ELISA: 1:5000
Other Names:
G0/G1 switch regulatory protein 3, G0S3, Protein fosB
Specificity Sensitivity:
FosB (Phospho-Ser27) antibody detects endogenous levels of FosB only when phosphorylated at serine 27.
References:
Martin-Gallardo A., Nat. Genet. 1:34-39(1992).
Heximer S.P., DNA Cell Biol. 15:1025-1038(1996).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
All Sites:
Human: Ser27; Mouse: Ser27
EGF + -
Immunofluorescence analysis of HuvEc cells, treated with EGF (200nM, 5mins), using FosB (Phospho-Ser27) antibody.