• Catalog Number:

  E1A0927A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human FAS around the phosphorylation site of tyrosine 291 (E-A-YP-D-T).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P25445

• Applications:

  IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  Apo-1, CD95, FasL receptor, TNFRSF6, TNR6, apoptosis-mediating surface antigen FAS, tumor necrosis factor receptor superfamily member 6

• Specificity Sensitivity:

  FAS (Phospho-Tyr291) antibody detects endogenous levels of FAS only when phosphorylated at tyrosine 291.

• References:

  Fred Ramsdell. Int. Immunol., Oct 1994; 6: 1545 - 1553. Rasheed Ahmad. J. Leukoc. Biol., Jan 2001; 69: 123. Gabriella Brunlid. Stem Cells, Jul 2007; 10.1634/stemcells.2006-0745. - ---------------------------------------------------------------------------------------------------------------------------------------------------------------

• All Sites:

  Human: Tyr291

P-peptide - +

Immunofluorescence analysis of COS7 cells, using FAS (Phospho-Tyr291) antibody. FAS (Phospho-Tyr291) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.