• Catalog Number:

  E1A0925A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human FAK around the phosphorylation site of tyrosine 397 (D-D-YP-A-E).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q05397

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  EC 2.7.10.2, FADK 1, FAK1, Focal adhesion kinase 1, PTK2, Protein- tyrosine kinase 2, pp125FAK

• Specificity Sensitivity:

  FAK (Phospho-Tyr397) antibody detects endogenous levels of FAK only when phosphorylated at tyrosine 397.

• References:

  Whitney G.S., DNA Cell Biol. 12:823-830(1993). Andre E., Biochem. Biophys. Res. Commun. 190:140-147(1993). Lee S.-T., Oncogene 8:3403-3410(1993).

• All Sites:

  Human: Tyr397; Mouse: Tyr428; Rat: Tyr397

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using FAK (Phospho-Tyr397) antibody. Western blot analysis of extracts from Jurkat cells, treated with Ca2+ (40nM, 30mins), using FAK (Phospho-Tyr397) antibody.