Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human FAK around the phosphorylation site of serine 910 (E-I-SP-P-P).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
FAK (Phospho-Ser910) antibody detects endogenous levels of FAK only when phosphorylated at serine 910.
References:
Whitney G.S., DNA Cell Biol. 12:823-830(1993).
Andre E., Biochem. Biophys. Res. Commun. 190:140-147(1993).
Lee S.-T., Oncogene 8:3403-3410(1993).
Matsuya M., J. Biol. Chem. 273:1003-1014(1998).
All Sites:
Human: Ser910; Mouse: Ser948; Rat: Ser913
P-peptide - +
P-peptide - +
Immunohistochemistry analysis of paraffin-embedded human brain tissue using FAK (Phospho-Ser910) antibody.
Immunofluorescence analysis of HepG2 cells, using FAK (Phospho-Ser910) antibody.