• Catalog Number:

  E1A0921A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Estrogen Receptor-α around the phosphorylation site of serine 305 (K-N-SP-L-A).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P03372

• Applications:

  IF: 1:100~1:500 ELISA: 1:20000

• Other Names:

  ER, ESR, ESR1, ESTR, ESTRA, Estradiol receptor, Estrogen receptor, NR3A1

• Specificity Sensitivity:

  Estrogen Receptor-α (Phospho-Ser305) antibody detects endogenous levels of Estrogen Receptor-α only when phosphorylated at serine 305.

• References:

  Greene G.L., Science 231:1150-1154(1986). Pink J.J., Nucleic Acids Res. 24:962-969(1996). Mungall A.J., Nature 425:805-811(2003). Pfeffer U., Cancer Res. 53:741-743(1993).

• All Sites:

  Human: Ser305; Mouse: Ser309

EGF + - Immunofluorescence analysis of HeLa cells, treated with EGF (200nM , 5mins), using Estrogen Receptor-α (Phospho-Ser305) antibody.