• Catalog Number:

  E1A0920A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human ERK8 around the phosphorylation site of threonine 175 and tyrosine 177 (A-V-TP-E-YP-V-A).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q8TD08

• Applications:

  WB: 1:500~1:1000 ELISA: 1:5000

• Other Names:

  EC 2.7.11.24, Extracellular signal-regulated kinase 8

• Specificity Sensitivity:

  ERK8 (Phospho-Thr175+Tyr177) antibody detects endogenous levels of ERK8 only when phosphorylated at threonine 175 and tyrosine 177.

• References:

  Wen-Liang Kuo. J. Biol. Chem., May 2004; 279: 23073 - 23081. Lorin M. Henrich. Mol. Cell. Biol., Sep 2003; 23: 5979 - 5988. Zhijian Qian. J. Biol. Chem., Jan 1999; 274: 1621.

• All Sites:

  Human: Thr175+Tyr177; Mouse: Thr176+Tyr178

Western blot analysis of extracts from Jurkat cells, using ERK8 (Phospho-Thr175+Tyr177) antibody.