• Catalog Number:

  E1A0918A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human ErbB2 around the phosphorylation site of threonine 686(K-Y-TP-M-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P04626

• Applications:

  IF: 1:100~1:500 ELISA: 1:5000

• Other Names:

  C-erbB-2, EC 2.7.10.1, Epidermal growth factor receptor-related protein, ErbB2, HER2, MLN 19, NEU, NEU proto-oncogene, NGL, Receptor protein-tyrosine kinase erbB-2, Receptor protein-tyrosine kinase erbB-2 precursor, Tyrosine kinase-type cell surface receptor HER2, kinase ErbB2, p185erbB2

• Specificity Sensitivity:

  ErbB2 (Phospho-Thr686) antibody detects endogenous levels of ErbB2 only when phosphorylated at threonine 686.

• References:

  Yamamoto T., Nature 319:230-234(1986). King C.R., Science 229:974-976(1985). Arcaro A., Mol. Cell. Biol. 20:3817-3830(2000). Olsen J.V., Cell 127:635-648(2006).

• All Sites:

  Human: Thr686; Mouse: Thr736; Rat: Thr688

P-peptide - + Immunofluorescence analysis of HuvEc cells, using ErbB2 (Phospho-Thr686) antibody.