Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Epo-R around the phosphorylation site of tyrosine 368 (D-T-YP-L-V).
Purification:
The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P19235
Applications:
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000
Other Names:
EPO-R, EPOR, Erythropoietin receptor precursor
Specificity Sensitivity:
Epo-R (Phospho-Tyr368) antibody detects endogenous levels of Epo-R only when phosphorylated at tyrosine 368.
P-peptide - +
Western blot analysis of extracts from K562 cells, using Epo-R (Phospho-Tyr368) antibody.
Immunofluorescence analysis of HepG2 cells, using Epo-R (Phospho-Tyr368) antibody.