• Catalog Number:

  E1A0914A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human EPHA2/3/4 around the phosphorylation site of tyrosine 588/596 (T-YP-V-D-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P29317/P29320/P54764

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000

• Other Names:

  EC 2.7.10.1, ECK, Ephrin type-A receptor 2 precursor, Epithelial cell kinase, MPK-5, SEK-2, SEK2, Tyrosine-protein kinase receptor ECK, kinase EphA2

• Specificity Sensitivity:

  EPHA2/3/4 (Phospho-Tyr588/596) antibody detects endogenous levels of EPHA2/3/4 only when phosphorylated at tyrosine 588/596.

• References:

  Lindberg R.A., Mol. Cell. Biol. 10:6316-6324(1990). Zhang Y., Mol. Cell. Proteomics 4:1240-1250(2005). Zhuang G., J. Biol. Chem. 282:2683-2694(2007).

• All Sites:

  Human: Tyr588/Tyr596/Tyr596; Mouse: Tyr589/Tyr596/Tyr596

P-peptide - + Western blot analysis of extracts from HepG2 cells, using EPHA2/3/4 (Phospho-Tyr588/596) antibody. Immunofluorescence analysis of HeLa cells, using EPHA2/3/4 (Phospho-Tyr588/596) antibody.