• Catalog Number:

  E1A0909A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human DNA-PK around the phosphorylation site of serine 2056 (Y-S-SP-Q-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 90%, Positives = 100%)

• SwissProtNo:

  P78527

• Applications:

  IHC: 1:50~1:100 ELISA: 1:10000

• Other Names:

  DNA- PKcs, DNA-dependent protein kinase catalytic subunit, DNPK1, EC 2.7.11.1, P460, PRKD, PRKDC, XRCC7, kinase DNA-PK

• Specificity Sensitivity:

  DNA-PK (Phospho-Ser2056) antibody detects endogenous levels of DNA-PK only when phosphorylated at serine 2056.

• References:

  Allan Y. Chen. AACR Meeting Abstracts, Mar 2004; 2004: 310. Laura J. Kienker. Nucleic Acids Res., Jul 2000; 28: 2752 - 2761. Sébastien Soubeyrand. Cancer Res., Mar 2003; 63: 1198 - 1201.

• All Sites:

  Human: Ser2056; Mouse: Ser2053

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using DNA-PK (Phospho-Ser2056) antibody. DNA-PK (Phospho-Ser2056) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.