• Catalog Number:

  E1A0906A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human DNA-PK around the phosphorylation site of threonine2647 (T-L-TP-Q-T).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P78527

• Applications:

  IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  DNA- PKcs, DNA-dependent protein kinase catalytic subunit, DNPK1, EC 2.7.11.1, P460, PRKD, PRKDC, XRCC7, kinase DNA-PK

• Specificity Sensitivity:

  DNA-PK (Phospho-Thr2647) antibody detects endogenous levels of DNA-PK only when phosphorylated at threonine 2647.

• References:

  Chung J., Korean J. Biochem. 27:193-197(1995). Owsianka A.M., Lahn B.T., Virology 257:330-340(1999). Lahn B.T., Science 278:675-680(1997). --------

• All Sites:

  Human: Thr2647

serum + - Immunofluorescence analysis of HuvEc cells, treated with serum (20%, 30mins), using DNA-PK (Phospho-Thr2647) antibody.