• Catalog Number:

  E1A0905A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MCM4 around the phosphorylation site of serine 54 (P-T-SP-P-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P33991

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Other Names:

  CDC21 homolog, DNA replication licensing factor MCM4, P1-CDC21

• Specificity Sensitivity:

  MCM4 (Phospho-Ser54) antibody detects endogenous levels of MCM4 only when phosphorylated at serine 54.

• References:

  Musahl C., Eur. J. Biochem. 230:1096-1101(1995). Livingston R.J., Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases. The MGC Project Team; Genome Res. 14:2121-2127(2004). --------

• All Sites:

  Human: Ser54; Mouse: Ser53

Western blot analysis of extracts from COS-7 cells, treated with nocodazole (1uM, 16hours), using MCM4 (Phospho-Ser54) antibody.