• Catalog Number:

  E1A0902A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human DDX3/DEAD-box Protein 3 around the phosphorylation site of threonine 322 (V-A-TP-P-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  O00571

• Applications:

  IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  D1PAS1 related sequence 2, D1PAS1-RS2, DBX, DDX3X, DEAD-box RNA helicase DEAD3, DEAD-box protein 3, DEAD-box, X isoform, DEAD3, EC 3.6.1.-, ERH, Embryonic RNA helicase, HLP2, Helicase-like protein 2

• Specificity Sensitivity:

  DDX3/DEAD-box Protein 3 (Phospho-Thr322) antibody detects endogenous levels of DDX3/DEAD-box Protein 3 only when phosphorylated at threonine 322.

• References:

  Chung J., Korean J. Biochem. 27:193-197(1995). Owsianka A.M., Virology 257:330-340(1999). Gevaert K., Proteomics 5:3589-3599(2005).

• All Sites:

  Human: Thr322; Mouse: Thr322; Rat: Thr323

P-peptide - +

serum + - Immunohistochemistry analysis of paraffin-embedded human brain tissue using DDX3/DEAD-box Protein 3 (Phospho-Thr322) antibody. Immunofluorescence analysis of HuvEc cells, treated with serum (20%, 30mins), using DDX3/DEAD-box Protein 3 (Phospho-Thr322) antibody.