• Catalog Number:

  E1A0901A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human DAPP1 around the phosphorylation site of tyrosine 139 (S-I-YP-E-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q9UHF2

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000

• Other Names:

  3' phosphoinositide-interacting SH2 domain containing protein, B lymphocyte adapter protein BAM32, Bam32, Dual adaptor for phosphotyrosine and 3-phosphoinositides 1, PHISH, dual adapter for phosphotyrosine and 3-phosphoinositides

• Specificity Sensitivity:

  DAPP1 (Phospho-Tyr139) antibody detects endogenous levels of DAPP1 only when phosphorylated at tyrosine 139.

• References:

  Dowler S., Biochem. J. 342:7-12(1999). Marshall A.J., J. Exp. Med. 191:1319-1332(2000). Zhang Q.-H., Genome Res. 10:1546-1560(2000).

• All Sites:

  Human: Tyr139; Mouse: Tyr139

P-peptide - + Western blot analysis of extracts from 293 cells, treated with Insulin (0.01U/ml, 2mins), using DAPP1 (Phospho-Tyr139) antibody. Immunofluorescence analysis of A549 cells, using DAPP1 (Phospho-Tyr139) antibody.