• Catalog Number:

  E1A0900A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human DAPK3 around the phosphorylation site of threonine 265 (R-M-TP-I-A).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  O43293

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  DAP kinase 3, DAP- like kinase, Death-associated protein kinase 3, Dlk, EC 2.7.11.1, ZIP-kinase, ZIPK

• Specificity Sensitivity:

  DAPK3 (Phospho-Thr265) antibody detects endogenous levels of DAPK3 only when phosphorylated at threonine 265.

• References:

  Kawai T., Mol. Cell. Biol. 18:1642-1651(1998). Murata-Hori M., BS Lett. 451:81-84(1999). Kawai T., Mol. Cell. Biol. 23:6174-6186(2003).

• All Sites:

  Human: Thr265; Mouse: Thr265

P-peptide - + Western blot analysis of extracts from HuvEc cells, using DAPK3 (Phospho-Thr265) antibody. Immunofluorescence analysis of A549 cells, using DAPK3 (Phospho-Thr265) antibody.