Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human CRYAB/Crystallin-α-B around the phosphorylation site of serine 59 (A-P-SP-W-F).
Purification:
The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
CRYAB/Crystallin-α-B (Phospho-Ser59) antibody detects endogenous levels of CRYAB/Crystallin-α-B only when phosphorylated at serine 59.
References:
Dubin R.A., Genomics 7:594-601(1990).
Iwaki A., Neurosci. Lett. 140:89-92(1992).
Kovalyov L.I., Electrophoresis 16:1160-1169(1995).
Scanlan M.J., Int. J. Cancer 83:456-464(1999)
All Sites:
Human: Ser59; Mouse: Ser59; Rat: Ser59
P-peptide - +
Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using CRYAB/Crystallin-α-B (Phospho-Ser59) antibody.