• Catalog Number:

  E1A0896A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CRYAB/Crystallin-α-B around the phosphorylation site of serine 45 (S-L-SP-P-F).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P02511

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Other Names:

  Alpha crystallin B chain, Alpha (B)-crystallin, CRYA2, P23, Rosenthal fiber component, crystallin, alphaB

• Specificity Sensitivity:

  CRYAB/Crystallin-α-B (Phospho-Ser45) antibody detects endogenous levels of CRYAB/Crystallin-α-B only when phosphorylated at serine 45.

• References:

  Dubin R.A., Genomics 7:594-601(1990). Iwaki A., Neurosci. Lett. 140:89-92(1992). The MGC Project Team; Genome Res. 14:2121-2127(2004).

• All Sites:

  Human: Ser45; Mouse: Ser45; Rat: Ser45

Western blot analysis of extracts from COS cells, treated with anisomycin (25ug/ml, 30mins), using CRYAB/Crystallin-α-B (Phospho-Ser45) antibody.