• Catalog Number:

  E1A0893A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human c-RAF around the phosphorylation site of serine 43(R-A-SP-D-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P04049

• Applications:

  WB: 1:500~1:1000 ELISA: 1:5000

• Other Names:

  C-RAF, C-Raf, CRAF, EC 2.7.11.1, Protein kinase raf 1, RAF proto-oncogene serine/threonine-protein kinase, RAF-1, RAF1, Raf proto-oncogene serine/threonine-protein kinase, Raf-1, Similar to murine leukemia viral (V-raf-1) oncogene homolog 1, kinase Raf1

• Specificity Sensitivity:

  c-RAF (Phospho-Ser43) antibody detects endogenous levels of c-RAF only when phosphorylated at serine 43.

• References:

  Morrison D.K., . Biol. Chem. 268:17309-17316(1993). King A.J., Nature 406:439-439(2000). Olsen J.V., Cell 127:635-648(2006). Matsuoka S., Science 316:1160-1166(2007).

• All Sites:

  Human: Ser43; Mouse: Ser43; Rat: Ser43

Western blot analysis of extracts from NIH/3T3cells, using c-RAF (Phospho-Ser43) antibody.