• Catalog Number:

  E1A0886A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human 53BP1 around the phosphorylation site of serine 25 (E-D-SP-Q-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q12888

• Applications:

  IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  P531, TP53B, TP53BP1, TRP53BP1, Tumor suppressor p53-binding protein 1, p53-binding protein 1

• Specificity Sensitivity:

  53BP1 (Phospho-Ser25) antibody detects endogenous levels of 53BP1 only when phosphorylated at serine 25.

• References:

  Irene M. Ward. Mol. Cell. Biol., Nov 2005; 25: 10079 - 10086. Fiona Pryde. J. Cell Sci., May 2005; 118: 2043 - 2055. Julio C. Morales. J. Biol. Chem., Apr 2003; 278: 14971 - 14977. .

• All Sites:

  Human: Ser25; Mouse: Ser25; Rat: Ser30

P-peptide - +

P-peptide - + Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using 53BP1 (Phospho-Ser25) antibody. Immunofluorescence analysis of NIH/3T3 cells, using 53BP1 (Phospho-Ser25) antibody.

53BP1 (Phospho-Ser25) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.