• Catalog Number:

  E1A0883A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Cytokeratin 8 around the phosphorylation site of serine 431 (L-T-SP-P-G).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P05787

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000

• Other Names:

  CK 8, CK8, CYK8, Cytokeratin endo A, K2C8, K8, KRT2-8, KRT8, Keratin, type II cytoskeletal 8, cytokeratin 8

• Specificity Sensitivity:

  Cytokeratin 8 (Phospho-Ser431) antibody detects endogenous levels of Cytokeratin 8 only when phosphorylated at serine 431.

• References:

  TA Hembrough, J. Cell Sci., Mar 1995; 108: 1071. MB Omary, J. Cell Biol., May 1992; 117: 583. Joachim Seipelt, J. Biol. Chem., Jun 2000; 275: 20084. CF Chou, J. Biol. Chem., Feb 1992; 267: 3901 - 3906.

• All Sites:

  Human: Ser431; Mouse: Ser436; Rat: Ser431

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Cytokeratin 8 (Phospho-Ser431) antibody. Western blot analysis of extracts from 293 cells, treated with EGF (200ng/ml, 30mins), using Cytokeratin 8 (Phospho-Ser431) antibody.

P-peptide - + Immunofluorescence analysis of HeLa cells, using Cytokeratin 8 (Phospho-Ser431) antibody.