• Catalog Number:

  E1A0880A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Cyclin E2 around the phosphorylation site of threonine 392 (I-M-TP-P-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%)

• SwissProtNo:

  O96020

• Applications:

  IF: 1:100~1:500 ELISA: 1:10000

• Other Names:

  CCNE2, CGE2, CYCE2, G1/S-Specific cyclin E2

• Specificity Sensitivity:

  Cyclin E2 (Phospho-Thr392) antibody detects endogenous levels of Cyclin E2 only when phosphorylated at threonine 392.

• References:

  Lisa K. Naeger. Cell Growth Differ., Jun 1999; 10: 413. Francisco Noya. J. Virol., Jul 2001; 75: 6121 - 6134. Suparna Mazumder. Mol. Cell. Biol., May 2007; 27: 3511 - 3520.

• All Sites:

  Human: Thr392; Mouse: Thr392

P-peptide - +

Immunofluorescence analysis of NIH/3T3 cells, using Cyclin E2 (Phospho-Thr392) antibody. Cyclin E2 (Phospho-Thr392) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.