Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human CSFR around the phosphorylation site of tyrosine 561 (N-S-YP-T-F).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
CSFR (Phospho-Tyr561) antibody detects endogenous levels of CSFR only when phosphorylated at tyrosine 561.
References:
Hampe A., Oncogene Res. 4:9-17(1989).
Andre C., Genomics 39:216-226(1997).
Liu T., J. Proteome Res. 4:2070-2080(2005).
All Sites:
Human: Tyr561; Mouse: Tyr559
P-peptide - +
Immunohistochemistry analysis of paraffin-embedded human brain using CSFR (Phospho-Tyr561) antibody.
Western blot analysis of extracts from HepG2 cells, using CSFR (Phospho-Tyr561) antibody.