• Catalog Number:

  E1A0873A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Coagulation Factor III around the phosphorylation site of serine 290 (E-N-SP-P-L).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P13726

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Other Names:

  CD142 antigen, F3, Tissue factor, coagulation factor III, thromboplastin

• Specificity Sensitivity:

  Coagulation Factor III (Phospho-Ser290) antibody detects endogenous levels of Coagulation Factor III only when phosphorylated at serine 290.

• References:

  Gregory S.G., Nature 441:315-321(2006). Muller Y.A., Biochemistry 33:10864-10870(1994). Muller Y.A., J. Mol. Biol. 256:144-159(1996).

• All Sites:

  Human: Ser290

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using Coagulation Factor III (Phospho-Ser290) antibody. Western blot analysis of extracts from RAW264.7 cells, treated with TNF (20ng/ml, 30mins), using Coagulation Factor III (Phospho-Ser290) antibody.