• Catalog Number:

  E1A0869A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human c-Jun around the phosphorylation site of threonine 231 (P-Q-TP-V-P).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P05412

• Applications:

  IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000

• Other Names:

  AH119, AP-1, AP1, Activator protein 1, Jun A, Proto-oncogene c-jun, RJG-9, V-jun avian sarcoma virus 17 oncogene homolog, c-Jun, p39

• Specificity Sensitivity:

  c-Jun (Phospho-Thr231) antibody detects endogenous levels of c-Jun only when phosphorylated at threonine 231.

• References:

  Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004). Rao S., J. Biol. Chem. 274:11115-11124(1999). Junius F.K., J. Biol. Chem. 271:13663-13667(1996).

• All Sites:

  Human: Thr231; Mouse: Thr234; Rat: Thr234

P-peptide - +

P-peptide - + Immunohistochemistry analysis of paraffin-embedded human testis tissue using c-Jun (Phospho-Thr231) antibody. Immunofluorescence analysis of HeLa cells, using c-Jun (Phospho-Thr231) antibody.