• Catalog Number:

  E1A0863A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CHOP around the phosphorylation site of serine 30 (V-L-SP-S-D).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P35638

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000

• Other Names:

  C/EBP-homologous protein, DDIT3, DNA-damage inducible transcript 3, GADD153, Growth arrest and DNA-damage-inducible protein GADD153

• Specificity Sensitivity:

  CHOP (Phospho-Ser30) antibody detects endogenous levels of CHOP only when phosphorylated at serine 30.

• References:

  Park J.S., Gene 116:259-267(1992). Crozat A., Nature 363:640-644(1993). Sjoeblom T., Science 314:268-274(2006).

• All Sites:

  Human: Ser30; Mouse: Ser30

Western blot analysis of extracts from Jurkat cells, treated with PMA (125ug/ml, 30mins), using CHOP (Phospho-Ser30) antibody.