• Catalog Number:

  E1A0862A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Chk1 around the phosphorylation site of serine 301 (D-F-SP-P-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  O14757

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:40000

• Other Names:

  CHEK1, Chk1, EC 2.7.11.1, kinase Chk1

• Specificity Sensitivity:

  Chk1 (Phospho-Ser301) antibody detects endogenous levels of Chk1 only when phosphorylated at serine 301.

• References:

  Sanchez Y., Science 277:1497-1501(1997). Shieh S.-Y., Genes Dev. 14:289-300(2000). Uensal-Kacmaz K., Mol. Cell. Biol. 25:3109-3116(2005). Matsuoka S., Science 316:1160-1166(2007).

• All Sites:

  Human: Ser301

P-peptide - + Western blot analysis of extracts from 293 cells, using Chk1 (Phospho-Ser301) antibody. Immunofluorescence analysis of HeLa cells, using Chk1 (Phospho-Ser301) antibody.