• Catalog Number:

  E1A0850A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CD50/ICAM-3 around the phosphorylation site of serine 518 (S-G-SP-Y-H).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P32942

• Applications:

  WB: 1:500~1:1000 ELISA: 1:40000

• Other Names:

  CD50 antigen; CDw50; ICA3; ICAM-R; ICAM3; Intercellular adhesion molecule-3 precursor

• Specificity Sensitivity:

  CD50/ICAM-3 (Phospho-Ser518) antibody detects endogenous levels of CD50/ICAM-3 only when phosphorylated at serine 518.

• References:

  Fawcett J., Nature 360:481-484(1992). Vazeux R., Nature 360:485-488(1992). de Fougerolles A.R., J. Exp. Med. 177:1187-1192(1993).

• All Sites:

  Human: Ser518

Western blot analysis of extracts from HepG2 cells treated with TNF, using CD50/ICAM-3 (Phospho-Ser518) antibody.