• Catalog Number:

  E1A0849A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CD5 around the phosphorylation site of tyrosine 453 (N-E-YP-S-Q).Puri-fication: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P06127

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000

• Other Names:

  LEU1, Lymphocyte antigen CD5, Lymphocyte glycoprotein Ly-1, Lymphocyte glycoprotein T1/Leu-1, Lyt-1, T-cell surface glycoprotein CD5 precursor

• Specificity Sensitivity:

  CD5 (Phospho-Tyr453) antibody detects endogenous levels of CD5 only when phosphorylated at tyrosine 453.

• References:

  Masanori Taniguchi, Blood, Feb 1998; 91: 1145 - 1151. David Kaplan, PNAS, Nov 2001; 98: 13850. SB Ebeling, J. Immunol., Dec 1993; 151: 6891. Glenn H. Cantor, J. Virol., Feb 2001; 75: 1689 - 1696.

• All Sites:

  Human: Tyr453; Mouse: Tyr452

P-peptide - + Western blot analysis of extracts from 293 cells, treated with PMA (125ng/ml, 30mins), using CD5 (Phospho-Tyr453) antibody. Immunofluorescence analysis of HepG2 cells, using CD5 (Phospho-Tyr453) antibody.