• Catalog Number:

  E1A0848A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CD45 around the phosphorylation site of serine 1007 (D-D-SP-D-S).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P08575

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000

• Other Names:

  EC 3.1.3.48, L-CA, LY-5, Leukocyte common antigen precursor, Leukocyte common antigen variant 4 precursor, Lymphocyte common antigen Ly-5, PTPRC, T200

• Specificity Sensitivity:

  CD45 (Phospho-Ser1007) antibody detects endogenous levels of CD45 only when phosphorylated at serine 1007.

• References:

  Streuli M., O J. 9:2399-2407(1990). Wu L., Mol. Cell. Biol. 22:2673-2686(2002). Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).

• All Sites:

  Human: Ser1007; Mouse: Ser996; Rat: Ser960

Western blot analysis of extracts from HeLa cells, treated with TNF (20ng/ml, 15mins), using CD45 (Phospho-Ser1007) antibody.