Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human CD44 around the phosphorylation site of serine 706(S-K-SP-Q-E).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
CD44 (Phospho-Ser706) antibody detects endogenous levels of CD44 only when phosphorylated at serine 706.
References:
H Haegel, J. Cell Biol., Sep 1993; 122: 1067.
TA Brown, J. Cell Biol., Apr 1991; 113: 207.
Stephen B. Fox, Cancer Res., Aug 1994; 54: 4539 - 4546.
G Koopman, J. Exp. Med., Apr 1993; 177: 897.
All Sites:
Human: Ser706; Mouse: Ser742
Western blot analysis of extracts from NIH/3T3 cells, treated with PMA (250ng/ml, 5mins), using CD44 (Phospho-Ser706) antibody.