• Catalog Number:

  E1A0846A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CD4 around the phosphorylation site of serine 433 (R-M-SP-Q-I).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P01730

• Applications:

  IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000

• Other Names:

  CD4, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4 precursor

• Specificity Sensitivity:

  CD4 (Phospho-Ser433) antibody detects endogenous levels of CD4 only when phosphorylated at serine 433.

• References:

  GR Harriman, J. Immunol., Oct 1990; 145: 2406. G Zauli, Blood, Sep 1994; 84: 1896 - 1905. Andrew Zloza, Blood, Sep 2003; 102: 2156 - 2164. David M. Rothstein, Int. Immunol., Jan 1993; 5: 409 - 418.

• All Sites:

  Human: Ser433

P-peptide - +

P-peptide - + Immunohistochemistry analysis of paraffin-embedded human brain tissue using CD4 (Phospho-Ser433) antibody. Immunofluorescence analysis of HepG2 cells, using CD4 (Phospho-Ser433) antibody.