• Catalog Number:

  E1A0845A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CD28 around the phosphorylation site of tyrosine 218 (A-A-YP-R-S).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P10747

• Applications:

  WB: 1:500~1:1000 ELISA: 1:40000

• Other Names:

  T-cell-specific surface glycoprotein CD28 precursor, TP44

• Specificity Sensitivity:

  CD28 (Phospho-Tyr218) antibody detects endogenous levels of CD28 only when phosphorylated at tyrosine 218.

• References:

  Aruffo A., Proc. Natl. Acad. Sci. U.S.A. 84:8573-8577(1987). Lee K.P., J. Immunol. 145:344-352(1990). Deshpande M., Hum. Immunol. 63:20-23(2002). --------

• All Sites:

  Human: Tyr218; Mouse: Tyr216

Western blot analysis of extracts from HepG2 cells, treated with nocodazole (1ug/ml, 16hours), using CD28 (Phospho-Tyr218) antibody.