• Catalog Number:

  E1A0843A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CD226/DNAM-1 around the phosphorylation site of serine 329 (T-F-SP-R-R).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q15762

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000

• Other Names:

  CD226, DNAM1, DNAX accessory molecule-1, PTA1, TLiSA1, adhesion glycoprotein, platelet and T cell activation antigen 1

• Specificity Sensitivity:

  CD226/DNAM-1 (Phospho-Ser329) antibody detects endogenous levels of CD226/DNAM-1 only when phosphorylated at serine 329.

• References:

  Shibuya A., Immunity 4:573-581(1996). Tahara-Hanaoka S., Int. Immunol. 16:533-538(2004). Pende D., Mol. Immunol. 42:463-469(2005).

• All Sites:

  Human: Ser329; Mouse: Ser326

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using CD226/DNAM-1 (Phospho-Ser329) antibody. Western blot analysis of extracts from COS7 cells, using CD226/DNAM-1 (Phospho-Ser329) antibody.

P-peptide - + Immunofluorescence analysis of A549 cells, using CD226/DNAM-1 (Phospho-Ser329) antibody.