• Catalog Number:

  E1A0842A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CD18/ITGB2 around the phosphorylation site of threonine 758 (S-A-TP-T-T).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human; Mouse; Rat

• SwissProtNo:

  P05107

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:40000

• Other Names:

  CD18; cell surface adhesion glycoproteins LFA- 1/CR3/p150,95 beta; complement receptor C3 beta; integrin beta-2; ITB2

• Specificity Sensitivity:

  CD18/ITGB2 (Phospho-Thr758) antibody detects endogenous levels of CD18/ITGB2 only when phosphorylated at threonine 578.

• References:

  Kishimoto T.K., Cell 48:681-690(1987). Weitzman J.B., FEBS Lett. 294-97-103(1991). Hattori M., Nature 405:311-319(2000).

• All Sites:

  Human: Thr758; Mouse: Thr760; Rat: Thr757

P-peptide - + Western blot analysis of extracts from COS cells, treated with EGF (200ng/ml, 30mins), using CD18/ITGB2 (Phospho-Thr758) antibody. Immunofluorescence analysis of HepG2 cells, using CD18/ITGB2 (Phospho-Thr758) antibody.