• Catalog Number:

  E1A0839A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CD136/RON around the phosphorylation site of serine 1394 (P-L-SP-E-P).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q04912

• Applications:

  WB: 1:500~1:1000 ELISA: 1:40000

• Other Names:

  CD136 antigen, CDW136, EC 2.7.10.1, MSP receptor, MST1R, Macrophage-stimulating protein receptor precursor, RON, STK, Stem cell-derived tyrosine kinase, kinase RON, p185-Ron

• Specificity Sensitivity:

  CD136/RON (Phospho-Ser1394) antibody detects endogenous levels of CD136/RON only when phosphorylated at serine 1394.

• References:

  Lily Lai, J. Immunol., Apr 1998; 160: 3861. Warren Casey, Physiol Genomics, Feb 2002; 8: 115. BENJAMIN P. C. CHEN, Physiol Genomics, Oct 2001; 7: 55. Christa E. Muller-Sieburg, Blood, Jun 2004; 103: 4111 - 4118.

• All Sites:

  Human: Ser1394; Mouse: Ser1372

Western blot analysis of extracts from HeLa cells, treated with TNF-a (20ng/ml, 2mins), using CD136/RON (Phospho-Ser1394) antibody.