• Catalog Number:

  E1A0835A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Caspase 3 around the phosphorylation site of serine 150 (C-R-SP-L-T).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P42574

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000

• Other Names:

  Apopain precursor, CASP-3, CPP-32, CPP32, Caspase-3, Cysteine protease CPP32, EC 3.4.22.-, ICE3, SCA-1, SREBP cleavage activity 1, Yama protein

• Specificity Sensitivity:

  Caspase 3 (Phospho-Ser150) antibody detects endogenous levels of Caspase 3 only when phosphorylated at serine 150.

• References:

  Daniela Sciaky, J. Immunol., Apr 2000; 164: 3806. Randolph Hutter, Circulation, Apr 2004; 109: 2001 - 2008. Andreas Hartmann, PNAS, Mar 2000; 97: 2875. Keith F. Izban, Am. J. Pathol., May 1999; 154: 1439.

• All Sites:

  Human: Ser150; Mouse: Ser150; Rat: Ser150

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human lymph node tissue using Caspase 3 (Phospho-Ser150) antibody. Western blot analysis of extracts from Jurkat cells, treated with Etoposide (25μM, 60mins), using Caspase 3 (Phospho-Ser150) antibody.