• Catalog Number:

  E1A0834A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Caspase 9 around the phosphorylation site of serine 196(F-S-SP-L-H).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P55211

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Other Names:

  APAF-3, Apoptotic protease Mch-6, Apoptotic protease activating factor 3, CASP-9, Caspase-9, Caspase-9 precursor, Caspase9, EC 3.4.22.-, ICE-LAP6, ICE-like apoptotic protease 6, MCH6, RNCASP9

• Specificity Sensitivity:

  Caspase 9 (Phospho-Ser196) antibody detects endogenous levels of Caspase 9 only when phosphorylated at serine 196.

• References:

  Duan H., J. Biol. Chem. 271:16720-16724(1996). Srinivasula S.M., J. Biol. Chem. 271:27099-27106(1996). Shiozaki E.N., Mol. Cell 11:519-527(2003).

• All Sites:

  Human: Ser196

Western blot analysis of extracts from 293 cells, treated with Calyculin (50nM, 30mins), using Caspase 9 (Phospho-Ser196) antibody.