• Catalog Number:

  E1A0833A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Caspase 9 around the phosphorylation site of serine 144 (L-E-SP-L-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P55211

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  APAF-3, Apoptotic protease Mch-6, Apoptotic protease activating factor 3, CASP-9, Caspase-9, Caspase-9 precursor, Caspase9, EC 3.4.22.-, ICE-LAP6, ICE-like apoptotic protease 6, MCH6, RNCASP9

• Specificity Sensitivity:

  Caspase 9 (Phospho-Ser144) antibody detects endogenous levels of Caspase 9 only when phosphorylated at serine 144.

• References:

  Yuanming Hu, J. Biol. Chem., Dec 1998; 273: 33489. C.S. Thota, Biol Reprod, Dec 2005; 73: 1191 - 1198. Eric J. Vallender, Hum. Mol. Genet., Oct 2006; 15: 3034 - 3040.

• All Sites:

  Human: Ser144

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma using Caspase 9 (Phospho-Ser144) antibody. Western blot analysis of extracts from K562 cells, using Caspase 9 (Phospho-Ser144) antibody.