• Catalog Number:

  E1A0831A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CaMK4 around the phosphorylation site of threonine 196/200 (M-K-TP-V-C).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q16566

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000

• Other Names:

  CAM kinase-GR, CAMK4, CaMK IV, CaMKIV, Calcium/calmodulin-dependent protein kinase type IV catalytic chain, Calspermin, EC 2.7.11.17, KCC4, kinase CaMK4

• Specificity Sensitivity:

  CaMK4 (Phospho-Thr196/200) antibody detects endogenous levels of CaMK4 only when phosphorylated at threonine 196/200.

• References:

  Christine M. Kitsos, J. Biol. Chem., Sep 2005; 280: 33101 - 33108. Joy Y. Wu, Mol. Cell. Biol., Sep 2001; 21: 6066 - 6070. Joy Y. Wu, Endocrinology, Dec 2000; 141: 4777. Stephen L. Wang, Cell Growth Differ., Jul 2001; 12: 351.

• All Sites:

  Human: Thr200; Mouse: Thr196; Rat: Thr196

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using CaMK4 (Phospho-Thr196/200) antibody. Western blot analysis of extracts from K562 cells, treated with H2O2 (100uM, 30mins), using CaMK4 (Phospho-Thr196/200) antibody.

P-peptide - + Immunofluorescence analysis of HeLa cells, using CaMK4 (Phospho-Thr196/200) antibody.