• Catalog Number:

  E1A0830A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CaMK1-α around the phosphorylation site of threonine 177 (L-S-TP-A-C).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q14012

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000

• Other Names:

  CAM kinase I, CAMK1, Calcium/calmodulin-dependent protein kinase type I, EC 2.7.11.17, KCC1, KCC1A, kinase CaMK1-alpha

• Specificity Sensitivity:

  CaMK1-α (Phospho-Thr177) antibody detects endogenous levels of CaMK1-α only when phosphorylated at threonine 177.

• References:

  Haribabu B., EMBO J. 14:3679-3686(1995). Gevaert K., Nat. Biotechnol. 21:566-569(2003). Hsu L.-S., J. Biol. Chem. 276:31113-31123(2001).

• All Sites:

  Human: Thr177; Mouse: Thr177; Rat: Thr177

Western blot analysis of extracts from K562 cells and Jurkat cells all treated with insulin (0.01U/ml, 15mins), using CaMK1-α (Phospho-Thr177) antibody.