• Catalog Number:

  E1A0829A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Calmodulin around the phosphorylation site of threonine 79 and serine 81 (K-D-TP-D-SP-E-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P62158

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000

• Other Names:

  CALM, CALM1, CALM2, CALM3, CAM, CAM1, CAM2, CAM3, CAMB, CAMC

• Specificity Sensitivity:

  Calmodulin (Phospho-Thr79+Ser81) antibody detects endogenous levels of Calmodulin only when phosphorylated at threonine 79 and serine 81.

• References:

  DM Roberts, PNAS, Sep 1992; 89: 8394. Whaseon Lee-Kwon, Am J Physiol Renal Physiol, Jan 2007; 292: F404 - F414. Roy A. Johanson, J. Neurosci., Apr 2000; 20: 2860. Ji Young Choi, J. Biol. Chem., Jun 2002; 277: 21630 - 21638.

• All Sites:

  Human: Thr79+Ser81; Mouse: Thr79+Ser81; Rat: Thr79+Ser81

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using Calmodulin (Phospho-Thr79+Ser81) antibody. Western blot analysis of extracts from Jurkat cells, treated with Insulin (0.01U/ml, 15mins), using Calmodulin (Phospho-Thr79+Ser81) antibody.

P-peptide - + Immunofluorescence analysis of HepG2 cells, using Calmodulin (Phospho-Thr79+Ser81) antibody.