• Catalog Number:

  E1A0827A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human C/EBP-ε around the phosphorylation site of threonine 74 (P-G-TP-P-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  Q15744

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000

• Other Names:

  C/EBP epsilon, CCAAT/enhancer binding protein epsilon, CEBPE

• Specificity Sensitivity:

  C/EBP-ε (Phospho-Thr74) antibody detects endogenous levels of C/EBP-ε only when phosphorylated at threonine 74.

• References:

  Arati Khanna-Gupta. PNAS, Jul 2001; 98: 8000. Dazhong Zhuang. J. Biol. Chem., Apr 2006; 281: 10745 - 10751. Julie A. Lekstrom-Himes. Stem Cells, Mar 2001; 19: 125 - 133. Ryuya Yamanaka. PNAS, Nov 1997; 94: 13187.

• All Sites:

  Human: Thr74

Western blot analysis of extracts from HUVEC cells, treated with UV (15mins), using C/EBP-ε (Phospho-Thr74) antibody.