• Catalog Number:

  E1A0825A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human C/EBP-α around the phosphorylation site of threonine 226 (H-P-TP-P-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P49715

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Other Names:

  C/EBP alpha, CCAAT/enhancer binding protein alpha, CEBPA

• Specificity Sensitivity:

  C/EBP-α (Phospho-Thr226) antibody detects endogenous levels of C/EBP-α only when phosphorylated at threonine 226.

• References:

  Antonson P., Biochem. Biophys. Res. Commun. 215:106-113(1995). Swart G.W.M., Biol. Chem. Hoppe-Seyler 378:373-379(1997). Choi B.H., J. Biol. Chem. 274:2858-2865(1999).

• All Sites:

  Human: Thr226; Mouse: Thr222; Rat: Thr222

Western blot analysis of extracts from COS7 cells, treated with EGF (200ng/ml, 30mins), using C/EBP-α (Phospho-Thr226) antibody.