• Catalog Number:

  E1A0823A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human B-Myb around the phosphorylation site of serine 577/581 (R-R-SP-P-I).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P10244

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:40000

• Other Names:

  BMYB, MYBB, MYBL2, Myb-related protein B

• Specificity Sensitivity:

  B-Myb (Phospho-Ser577/581) antibody detects endogenous levels of B-Myb only when phosphorylated at serine 577/581.

• References:

  Nomura N., Nucleic Acids Res. 16:11075-11089(1988). Deloukas P., Nature 414:865-871(2001). Olsen J.V., Cell 127:635-648(2006). Litovchick L., Mol. Cell 26:539-551(2007).

• All Sites:

  Human: Ser577; Mouse: Ser581; Rat: Ser582

P-peptide - + Western blot analysis of extracts from K562 cells, using B-Myb (Phospho-Ser577/581) antibody. Immunofluorescence analysis of HeLa cells, t using B-Myb (Phospho-Ser577/581) antibody.