• Catalog Number:

  E1A0822A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human BAD around the phosphorylation site of serine 134 (P-K-SP-A-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q92934

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000

• Other Names:

  BAD, BBC6, BCL2L8, Bcl- XL/Bcl-2 associated death promoter, Bcl-2 binding component 6, Bcl-2-like 8 protein, Bcl-xL/Bcl-2 associated death promoter, Bcl2-antagonist of cell death

• Specificity Sensitivity:

  BAD (Phospho-Ser134) antibody detects endogenous levels of BAD only when phosphorylated at serine 134.

• References:

  Ottilie S., J. Biol. Chem. 272:30866-30872(1997). Wang H.-G., Cell 87:629-638(1996). Petros A.M., Protein Sci. 9:2528-2534(2000).

• All Sites:

  Human: Ser134; Mouse: Ser170; Rat: Ser171

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using BAD (Phospho-Ser134) antibody. Western blot analysis of extracts from mouse liver cells, using BAD (Phospho-Ser134) antibody.

P-peptide - + Immunofluorescence analysis of HeLa cells, using BAD (Phospho-Ser134) antibody.