• Catalog Number:

  E1A0819A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human ATF2 around the phosphorylation site of serine 480 (P-S-SP-Q-S).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P15336

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000

• Other Names:

  ATF2, Activating 2, CREB2, CREBP1, Cyclic-AMP-dependent ATF-2, HB16, MXBP protein, cAMP response element binding protein CRE- BP1

• Specificity Sensitivity:

  ATF2 (Phospho-Ser480) antibody detects endogenous levels of ATF2 only when phosphorylated at serine 480.

• References:

  Maekawa T., EMBO J. 8:2023-2028(1989). Yang L., J. Immunol. 158:2522-2525(1997). Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).

• All Sites:

  Human: Ser480; Mouse: Ser480; Rat: Ser480

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using ATF2 (Phospho-Ser480) antibody. Western blot analysis of extracts from HUVEC cells, treated with Anisomycin (1ng/ml, 15mins), using ATF2 (Phospho-Ser480) antibody.