• Catalog Number:

  E1A0818A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human APC around the phosphorylation site of serine 2054 (K-P-SP-R-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P25054

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000

• Other Names:

  APC, APC protein, Adenomatous polyposis coli protein, DP2.5, mAPC

• Specificity Sensitivity:

  APC (Phospho-Ser2054) antibody detects endogenous levels of APC only when phosphorylated at serine 2054.

• References:

  Matthew Bjerknes, Am. J. Pathol., Jun 1999; 154: 1831. T Maltzman, Carcinogenesis, Dec 1997; 18: 2435 - 2439. Jean Schneikert, Hum. Mol. Genet., Jan 2007; 16: 199 - 209. Dhaval M. Patel, J. Immunol., Nov 1999; 163: 5201.

• All Sites:

  Human: Ser2054; Mouse: Ser2053; Rat: Ser2053

Western blot analysis of extracts from HUVEC cells, treated with PMA (125ng/ml, 30mins), using APC (Phospho-Ser2054) antibody.